The ongoing war in Ukraine is associated with a number of negative and long-term macroeconomic consequences for Poland and other countries both in the area of trade and maintaining supply chains, as well as the security of energy supplies. Many entities in the agri-food sector are affected by this impact. The aim of the article is to assess the effects of the war in Ukraine on Polish agri-food trade. The paper presents the production and trade potential of Ukraine, defines trends in Polish agri-food trade with Ukraine after February 24, 2022, and identifies the main effects for Polish agri-food trade resulting from the ongoing armed conflict in Ukraine. Several research methods were used in the article, ranging from a literature review and document analysis, through a statistical and descriptive method of production and trade data analysis, to competitiveness indices: the index of comparative advantages (RCA) and trade coverage ratio (TC). The research shows that in 2022 Polish imports of agri-food products from Ukraine increased almost three times in terms of value, as compared to 2021, reaching EUR 2.8 billion, which increased the competitive pressure on the internal market. It has also been proven that only part of agri-food products imported from Ukraine is subject to mutual Polish-Ukrainian competition on the EU market. This applies to products such as wheat, poultry meat, birds’ eggs, frozen fruit, but also highly processed food products, including confectionery and pastry. Further development of trade in agri-food products with Ukraine will be determined primarily by the situation at the front, the supply of goods for export, the possibility of exporting Ukrainian goods through the Black Sea ports, and the permeability of land solidarity routes. Data shows that in 2022 the yields of cereals, legumes, and oilseeds in Ukraine decreased by 34%, which should also translate into decreased exports.