The aim of the study was to present agricultural market output as a criterion for using agricultural potential in various regions of Poland. The analysis was conducted taking into account selected indicators characterizing the natural, agrotechnical, organizational, and economic conditions of agriculture in individual voivodeships. The average for Poland was used as the basis for comparisons. The basic sources of information were the statistical data of Statistics Poland, the research results of the Institute of Soil Science and Plant Cultivation – State Research Institute in Puławy, and the research results presented in the literature. According to our hypothesis, the environmental, agrotechnical, organizational, and economic conditions determine the level and structure of agricultural market output in Poland as a criterion for using the agricultural potential in the regions. The research conducted so far shows that the impact of individual groups of conditions is clearly differentiated and visible in the production specialization and their share in agricultural market output in Poland. Regional diversification of agricultural market output in Poland should be basis for directing scientific research and advisory activities. It also reflects the regional differentiation of the effects of the EU Common Agricultural Policy.